PP202 Stem cell-like subtype from developmental hierarchies of fetal liver shows clinical relevance with prognostic significance in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is lethal malignancy showing high relapse rates after curative resection in early-stage. Genomic features of stem cell-like cancer cells contributing aggressive tumor biology HCC remains unclear. The aim this study to understand underlying associated with stemness apply precise therapeutic strategies for resectable early-stage hepatocellular carcinoma. Using human fetal liver signatures, Multi-omics dataset from multiple clinical cohorts were analyzed comprehensively reveal molecular mechanisms as well potential biomarkers enhance efficacy targeted therapy or immunotherapy subtypes. patients predicted the hepatic cell (HS) subtype showed including large size, AFP, vascular invasion, and extrahepatic metastasis worst prognosis early recurrence even oncogenic pathways terms cycle, epithelial-mesenchymal transition, TGF-beta pathway highly upregulated HS subtype. Higher mutations TP53, RB1 PTEN deletion significantly identified We also demonstrated subtype-specific tissue serum Predicted responders lower subtypes due higher accumulation tumor-associated macrophages myleolid-derived suppressor cells. response multi-tyrosine kinase inhibitors, especially sorafenib lenvatinib. Stem not only a rate but HCC. each regarding molecular-targeted treatment. Our findings may offer theoretical foundation biomarker-based trials new approaches patients.